Figure 2.

Comparison of Vγ2Vδ2 T cell-mediated cytotoxicity induced by conventional N-BPs (left panels) or their fluorine-containing analogs (right panels). 786–0 renal carcinoma cells were either not treated (Δ) or pretreated with second generation N-BPs (1, 2, or 3) at 1000 μM (○) or 3000 μM (●) or their fluorine-containing analogs (6, 7 or 8) at 1000 μM (○) or 3000 μM (●) or third generation N-BPs (4 or 5) at 300 μM (○) or 1000 μM (●) or their fluorine-containing analogs (9 or 10) at 1000 μM (○) or 3000 μM (●). 786–0 renal carcinoma cells were pretreated with the N-BPs at 37^oC for 2 h. The chelate-forming BM-HT prodrug reagent was then added to 25 μM and the cells incubated at 37^oC for an additional 15 min. After being washed with RPMI1640 medium, the cells were cultured with Vγ2Vδ2 T cells for 40 min. Supernatants from the cultures were then harvested and mixed with Eu³⁺ solution. Specific lysis was determined by measuring time-resolved fluorescence using a PHERAStar multiplate reader. Data show mean ± SD and are representative of three independent experiments.