Figure 3.

Vγ2Vδ2 T cell-mediated cytotoxicity induced by tetrakispivaloxylmethyl-1-fluoro-2-(1H-imidazol-1-yl)ethylidene-1,1-bisphosphonate (11). 786–0 and UOK121 renal carcinoma cells were pretreated with 0 μM (Δ), 1 μM (○) or 5 μM (●) of 11 at 37^oC for 2 h. Similarly, EJ-1 bladder carcinoma cells and U937 histiocytic leukemia cells were pretreated with 0 μM (Δ), 1.25 μM (▲), 2.5 μM (○) or 5 μM (●) of 11. The cells were further treated with 25 μM of BM-HT at 37^oC for 15 min and specific lysis by Vγ2Vδ2 T cells determined as in Fig. 2. Data show mean ± SD and are representative of three independent experiments.