Skip to main content
. 2019 Aug 15;117(6):1156–1165. doi: 10.1016/j.bpj.2019.08.009

Figure 1.

Figure 1

Deterministic model of the adaptive response. (A) Schematic of the model is shown; Ada is expressed at a low basal level in undamaged cells. MMS treatment creates DNA methylation damage that converts Ada to meAda. Transcription of ada is activated by meAda binding to the PAda promoter. Both Ada and meAda molecules are diluted because of cell growth and division. Additionally, meAda gets inactivated by degradation or autorepression. (B) Shown is the cell average steady-state expression of Ada after constant treatment with different doses of MMS for 20 cell generations (mean ± SD). The curve shows the analytical steady-state solution of the model for the total Ada abundance (Ada + meAda + inAda). (C) Shown is the cell average response when MMS was added at time 0 and Ada abundance was measured by time-lapse fluorescence microscopy. The model curves of total Ada abundance were generated by numerically solving the rate equations for different MMS concentrations. (D) Shown is the cell average response and response deactivation upon the addition and removal of 500 μM MMS during time-lapse microscopy. The vertical line indicates the time of MMS removal. The numerical solution of the model shows total Ada and meAda abundances as solid and dashed lines, respectively. To see this figure in color, go online.