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. 2019 Aug 29;117(7):1352–1363. doi: 10.1016/j.bpj.2019.08.028

Figure 3.

Figure 3

Electrophysiology of ANAP incorporated full-length and ΔCT truncated Nav1.5 in HEK293T cells. (a) Representative TTX-sensitive capacitance-normalized current traces of wild-type Nav1.5, Nav1.5(ΔCT), Nav1.5(Q1475ANAP), or Nav1.5(Q1475ANAP/ΔCT) expressed in HEK293T is shown. All conditions were co-transfected with AnapRS-LeutRNACUA. Inset is the voltage protocol. (b) Average peak current density (pA/pF) of all conditions is shown. Error bars are ± SEM. Numbers above bars are the number of cells. ∗(left to right) two-tailed p = 8.5 × 10−5, 1.7 × 10−5, 0.02, 5.6 × 10−6, Student’s t-test. Peak currents and cell capacitances are in Fig. S1. (c) SSI of all constructs ± 10 μM ANAP is shown. Error bars are ± SEM. Inset is the voltage protocol. Numbers in legend refer to number of cells. V1/2: WT −64.6 ± 0.4 mV, Q1475ANAP −51.8 ± 0.7 mV, ΔCT −79.2 ± 0.4 mV, Q1475ANAP/ΔCT −59.7 ± 0.6 mV (d) Time to half-complete inactivation (t1/2, ms) of all constructs is shown. Error bars are ± SEM. Numbers above bars are the number of cells. ∗(left to right) two-tailed p = 0.0009, 0.0002, Student’s t-test. (e) High-gain TTX-sensitive capacitance-normalized traces of all constructs using same voltage protocol as in (a) are shown. Arrow indicates late sodium current (INa,L). INa,L (% peak current, late current density): WT (0.3%, 1 pA/pF), Q1475ANAP (1.3%, 0.9 pA/pF), ΔCT (0.46%,1.2 pA/pF), Q1475ANAP/ΔCT (3.1%, 0.8 pA/pF). To see this figure in color, go online.