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. 2019 Aug 30;117(7):1285–1300. doi: 10.1016/j.bpj.2019.08.030

Figure 11.

Figure 11

(A) FRAP data for LAF-1 at 80 mM NaCl (open black circles) using Rdrop/Rbleach = 1 fitted using the 3D finite model (purple dashed line). (Inset) Fitting to the 3D finite model results in a two-orders-of-magnitude-lower apparent D compared to that measured for Rdrop/Rbleach ≫1 using the 3D infinite nonstep model (i.e., 1.2 ± 0.3 × 10−5 and R2 = 0.97 vs. 1.7 ± 0.5 × 10−3μm2/s). (B) Fitting the data to the 3D finite model with interface resistance (black dotted line) yields k = 0.08 ± 0.01 μm/s, and R2 = 0.71. Typical error bars are shown and represent the SD of five replicates. To see this figure in color, go online.