Figure 9.

(A) xz image of LAF-1 droplets before and immediately after bleaching obtained using an ROI diameter equal to 3 μm, where R_drop/R_bleach varies from 1 to 10. (B) Measured apparent diffusion coefficients for each value of R_drop/R_bleach using the 3D infinite model with a nonstep initial condition are shown. (C) Measured apparent diffusion coefficient scaled by diffusion coefficient measured in large droplets in microfluidic devices, D_infinite = 0.0017 ± 0.0005 μm²/s, is shown. Blue dashed line and shaded blue area indicate average diffusion coefficient and SD, respectively, measured in large droplets in microfluidic devices. Typical error bars are shown and represent SD of at least three replicates. To see this figure in color, go online.