Skip to main content
. 2019 Sep 20;16(11):1412–1423. doi: 10.7150/ijms.34398

Figure 3.

Figure 3

Synergistic effects of TRAIL and bortezomib in SNU-216. (A) SNU-216 was treated with TRAIL (12.5 ng/ml) in combination with bortezomib (22.75 nM) for 24 h and 48 h. Cell viability was analyzed by MTT assay and relative cell viability was calculated against untreated control. Results are presented as the mean ± SE of three independent experiments. P values were calculated from one-way ANOVA. (B) The cells were co-treated with TRAIL (0.0, 12.5, 25.0 and 50 ng/ml) and bortezomib (0.0, 5.7, 11.4, 22.8, 45.5 and 91.0 nM) in a range of concentration for 24 h and 48 h. Cell viability was measured by MTT assay and synergism of drug combinations was analyzed with isobologram. (C) Apoptotic cells were determined by flow cytometry of Annexin V/PI staining. Lower panel shows fractions of Annexin V positive and negative cells, respectively. P values were calculated from one-way ANOVA. (D) Western blot was used to detect the activation of caspases-3,-8 and -9. The data shown are representatives of triple experiments (C and D).