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. Author manuscript; available in PMC: 2020 Oct 2.
Published in final edited form as: Matter. 2019 Sep 11;1(4):926–944. doi: 10.1016/j.matt.2019.07.019

Figure 4. In vitro 3D encapsulation of W-20–17 cells and mouse calvarial bone sutures inside adhesive hydrogels.

Figure 4.

(A) Representative live/dead images of W-20–17 cells encapsulated within bioadhesives hydrogels with and without AMP after 1 and 5 days. (B) Quantification of viability of W-20–17 cells incorporated within hydrogels without (control) and with AMP (GelAMP) using live/dead assays on days 1, 3, and 5 post encapsulation. (C) Representative phalloidin (green)/DAPI (blue) stained images of cell-laden bioadhesive with and without AMP after 1 and 5 days. (D) Quantification of metabolic activity of W-20–17 cells encapsulated in hydrogels after 1, 3, and 5 days. (E) Schematic diagram of the extraction and encapsulation of mouse calvarial bone sutures in 3D hydrogel network. (F) Representative images of calvarial bone sutures encapsulated within 7 % and 15% (w/v) bioadhesives to visualize growth and diffusion of cells at days 10, 20 and 30 post encapsulation. (G) Quantification of metabolic activity of migratory stromal cells from encapsulated bone sutures. Hydrogels were formed at 120 sec visible light exposure time (** p < 0.01, *** p < 0.001), **** p < 0.0001).