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Immunohistochemical analysis of engineered constructs in vivo. Localization and content of type I and II collagen in the constructs were examined 8 weeks after transplantation into nude mice. (a): Immunostaining for type I and II collagen. Scale bars = 100 μm (b): Protein synthesis of type I and II collagen, examined by ELISA. Data are expressed as mean ± standard deviation. Statistical significance was evaluated using Dunnett's test. *p < 0.05 vs. direct group.
