Fig. 6.

Chondrogenesis of engineered constructs in vivo (beagle). The constructs were fabricated with P0 canine auricular chondrocytes, atelocollagen (Koken), and PLLA scaffolds, and then transplanted subcutaneously into the back of beagles. Eight weeks after transplantation, the constructs were evaluated histologically and biochemically. (a): Morphological features of the constructs. Scale on the ruler = 500 μm. (b): Toluidine blue staining. Scale bars = 1 mm. (c): GAG accumulation examined by Alcian blue binding assay. Data are expressed as mean ± standard deviation. Statistical significance was evaluated using Dunnett's test. *p < 0.05 vs. the direct group.