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. 2019 Oct 22;10:1317. doi: 10.3389/fpls.2019.01317

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Copyright © 2019 Díaz-Valle, López-Calleja and Alvarez-Venegas

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transgenerational transcript levels of common bean genes. (A) Transcript levels of genes involved in plant defense. (A) PvWRKY33; (B) PvPAL2; (C) PvERF6. F0 plants were self-pollinated and grown to seed set to generate the F1 progeny. Seeds from the F1 progenies were germinated and exposed to the pathogen only, without rhizobacteria treatment. Samples were obtained at 16, 18, and 22 dag. Data were normalized to the actin11 (PvActin11) and tubulin (PvTUB, see Figure SF4 ) reference genes. (B) Transcript levels of the PvAAO3 gene involved in ABA synthesis. Data were normalized to the actin11 (PvActin11) and tubulin (PvTUB) reference genes. Data are mean ± SD from three independent experiments (n = 3). Statistical significance was determined using two-way ANOVA followed by Tukey’s multiple comparison test, p < 0.05. Dag, days after germination.