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. 2019 Oct 22;10:1317. doi: 10.3389/fpls.2019.01317

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Copyright © 2019 Díaz-Valle, López-Calleja and Alvarez-Venegas

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcript levels of genes involved in plant hormone biosynthesis. (A) PvAAO3; (B) PvAMI1; (C) PvNIT1; (D) PvBROX2.2. Plants were primed with R. etli, followed by inoculation with PspNPS3121 (Re/Ps), inoculated only with the symbiont (Re/- ; and water, Re/H2O), infected only (-/Ps), or neither primed nor inoculated (control, -/-). Data were normalized to the Actin11 (PvActin11) and Tubulin (PvTUB, see Supplementary Figure SF3 ) reference genes. Data represent mean ± SD from three independent experiments (n = 3). Statistical significance was determined using two-way ANOVA followed by Tukey’s multiple comparison test, p < 0.05. Dag: days after germination. Means with the same letter are not significantly different.