The profiles of TAG, TFAs, and LDs in C. zofingiensis under ND conditions
Time course of TAG content (A), TFAs content (B), and TAG/TFAs ratio (C) in response to ND. Fatty acid composition of TAG (D) and TFAs (E) at 0 and 48 h of ND. (F) Fluorescence microscopy of cells stained with the neutral lipid dye Bodipy. LDs stained by Bodipy are shown in green, whereas red indicates chlorophyll autofluorescence. Data in A–E are expressed as mean ± SD (n = 3). An asterisk indicates a significant difference at the specified time point compared to 0 h (P < 0.05; t-test). TAG, triacylglycerol; TFAs, total fatty acids; LD, lipid droplet; ND, nitrogen deprivation; DW, dry weight.