Figure 2.

Quality evaluation of the isolated LD fraction

A. Microscopic image of isolated LDs under DIC and fluorescence mode stained with Bodipy. B. Absorption spectra of acetone extracts obtained from ND-induced WC and purified LD fraction (48 h of ND). C. TLC analysis of lipid extracts from WC (0 h and 48 h of ND) and purified LD fraction (48 h of ND). Arrows on the top and bottom indicate TAG and polar lipids, respectively. D. The percentage of polar lipids in TFA of WC (0 h and 48 h of ND) and in purified LD fraction (48 h of ND). Data are expressed as mean ± SD (n = 3). Groups that are significantly different from each other are marked with different letters (P < 0.05; one-way ANOVA Tukey’s honestly significant difference test). E. Western blotting analysis for the evaluation of contamination in the LD fraction (two replicates, R1 and R2). RBCL, AOX, and BIP were used as the markers for chloroplast, mitochondria, and ER, respectively. DIC, differential interference contrast; WC, whole cell; RBCL, RuBisCO large subunit; AOX, alternative oxidase; BIP, binding immunoglobulin protein.