Figure 5.

Heterologous expression of C. zofingiensis MLDP in C. reinhardtii

A. Microscopic visualization of LDs (indicated by green signal) in various Chlamydomonas strains of CC-5325, Crmldp, Crmldp/CzMLDP, and CC-5325/CzMLDP. B. Contents of TAG and TFAs, TAG/TFAs ratio, and fatty acid profile of TAG. Algal cells were grown under ND conditions for 48 h. Data are expressed as mean ± SD (n = 3). Groups that are significantly different from each other are marked with different letters, whereas no significant difference was found between groups labelled with the same letters (P < 0.05; one-way ANOVA Tukey’s honestly significant difference test). Cz, C. zofingiensis; Cr, Chlamydomonas reinhardtii; Crmldp/CzMLDP, the Crmldp mutant strain expressing CzMLDP gene; CC-5325/CzMLDP, the wild-type strain CC-5325 expressing CzMLDP gene.