Table 2.
The content and fatty acid composition of TAG in yeast as affected by the expression of LD proteins
|
LD protein |
TAG content (µg/OD600) |
Fatty acid composition of TAG (%) |
|||
|---|---|---|---|---|---|
| C16:0 | C16:1 | C18:0 | C18:1 | ||
| Empty vector | 12.95 ± 0.58 | 29.40 ± 0.85 | 33.45 ± 1.38 | 14.87 ± 0.57 | 22.29 ± 0.17 |
| #1 (MLDP) | 16.71 ± 1.03* | 32.43 ± 0.95* | 29.72 ± 1.16* | 17.26 ± 0.76* | 20.59 ± 1.22 |
| #2 (FUP) | 10.74 ± 1.04 | 35.92 ± 0.72* | 28.57 ± 0.28* | 17.28 ± 0.27* | 18.22 ± 0.61* |
| #3 (Caleosin) | 16.62 ± 0.27* | 40.05 ± 1.63* | 23.32 ± 0.72* | 20.13 ± 0.26* | 16.50 ± 1.38* |
| #4 (FUP) | 13.99 ± 0.34 | 39.77 ± 0.62* | 22.28 ± 2.15* | 20.73 ± 0.44* | 17.22 ± 2.18* |
| #5 (GULO) | 17.98 ± 0.97* | 35.25 ± 0.66* | 27.97 ± 1.30* | 17.11 ± 0.83* | 19.67 ± 0.30* |
| #6 (Caleosin) | 19.05 ± 0.99* | 37.54 ± 0.96* | 23.83 ± 4.00* | 20.45 ± 2.31* | 18.18 ± 1.12* |
| #8 (RDH) | 11.17 ± 0.83 | 31.48 ± 0.83 | 37.26 ± 1.11* | 11.31 ± 0.96* | 19.95 ± 0.54* |
| #13 (Lipase) | 20.08 ± 1.01* | 30.43 ± 0.47 | 30.59 ± 0.98 | 14.06 ± 1.31 | 24.92 ± 0.50 |
| #39 (Lipase) | 17.57 ± 0.61* | 28.66 ± 1.29 | 33.92 ± 1.20 | 12.78 ± 0.57* | 24.64 ± 0.94 |
Note: Data are expressed as mean ± SD (n = 3); Asterisk indicates the significant difference compared to the empty vector control (P < 0.05; t-test). See Dataset S1 for more information about the LD proteins shown in the first column. TAG content was expressed as µg/OD600 in yeast cells. MLDP, major lipid droplet protein; FUP, function unknown protein; GULO, l-gulonolactone oxidase; OD, optical density; RDH, retinol dehydrogenase.