Table 1.
Human primer sets used for qRT–PCR in tumours of patients
| Extracellular matrix components | ||
|---|---|---|
| Gene | Forward | Reverse |
| B2m | GAATTGCTATGTGTCTGGGT | TCTTCAAACCTCCATGATGCT |
| COL1A | AGAGGTTTCCCTGGCGA | ACCAGCATCACCCTTAGCA |
| COL3A | CTCAGGGTGTCAAGGGT | CAGGGTTTCCATCTCTTCCA |
| MMP2 | TGAGACCGCCATGTCCA | TCGCACACCACATCTTTCC |
| MMP9 | ACTACTGTGCCTTTGAGTCC | GCCAGTACTTCCCATCCT |
| TGF‐β3 | AACAATTCCTGGCGATACCT | GTAGTGAACCCGTTGATGTC |
| Vimentin | AAAGGAACCAATGAGTCCCT | GCAGGTCTTGGTATTCACGA |
| HIF‐1α | CTCATCAGTTGCACTCC | ATCCAAATCACCAGCATCCA |
B2m, β2‐microglobulin; COL1A, type I collagen; COL3A, type III collagen; HIF‐1α, hypoxia‐inducible factor‐1α; MMP2, matrix metalloproteinase 2; MMP9, matrix metalloproteinase 9; qRT–PCR, Real‐Time Quantitative Reverse Transcription PCR; TGF‐β3, transforming growth factor‐β3.