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Detection limits of the HSV1/HSV2, and IS61160 PCR assays. Curves were determined by probit analysis (95% probability detection). A, The LOD was estimated at 2.45 copies/µL for HSV1; B, 1.72 copies/µL for HSV2; and C, 2.54 copies/µL for IS6110 using the BCG strain containing a single target copy of IS6110. Dashed lines show 95% confidence interval for the analysis. BCG, bacillus Calmette‐Guerin; HSV, herpes simplex virus; LOD, limits of detection
