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Description of primers and probes
| Microorganisms | Primers’ sequence (5′ 3′) | Genome region amplified | Amplicon, bp |
|---|---|---|---|
| HSV1/HSV2 | |||
| Forward | AAGAGCCGCGTGTTGTTC | UL29 | 121 |
| Reverse | GTCCGAGGAGGATGTCCA | ||
| Probe | CCTACCAGAAGCCCGACAAG | ||
| Mycobacterium tuberculosis | |||
| Forward | CATGTCCGGAGACTCCAGTT | IS6110 | 66 |
| Reverse | GGTACCTCCTCGATGAACCA | ||
| Probe | AAAGGATGGGGTCATGTCAG | ||
HSV, herpes simplex virus.
