Table 2. MR techniques, key parameters, and site-specific parameter adjustments (red) at each site involved in harmonization: UEF, Melbourne and UCLA.

Harmonization step 1 was to tailor the sequences executable by each MRI system and account for the different field strengths and gradient performances. Step 2 was to tailor the sequence parameters to unify signal to noise ratio and contrast between different field strengths/sites. Flip angles were calculated to match the Ernst angle, repetition times (TR) to match the T1 relaxation, and echo times (TE) to match the T2 relaxation between sites. Differences in parameters between 7 T and 4.7 T are indicated in red.

	UEF	Melbourne	UCLA
	multi-slice T2 weighted fast spin echo (FSE): edema, anatomy
Method	TurboRARE	TurboRARE	TurboRARE
TR/TEeff	3400/45 ms	3400/49ms	3400/45 ms
echoes per excitation	8	8	8
resolution/slice thickness	117 × 117 μm/0.8mm	117 × 117 μm/0.8mm	117 × 117 μm/0.8mm
	*3D multi-echo gradient echo (MGE) 160μm³: SWI, T2 and anatomy**
Method	MGE 3D	MGE 3D	MGE 3D
TR/TEmax/flip/SW	66 ms/43 ms/16°/67kHz	66 ms/47ms/18°/67kHz	66 ms/43 ms/16°/67kHz
T2* - array: echoes	13	14	13
TEmin/ step /TEmax	2.73/3.1/43 ms	2.73/3.4/47ms	2.73/3.1/43 ms
Dummys	120 - > 5640 ms (5*T1)	120- > 5640 ms (> 5*T1)	120 - > 5640 ms (5*T1)
OVS pulses	4 OVS	4 OVS	4 OVS
Fat sat	Yes	Yes	Yes
	Diffusion-weighted imaging (DWI) 250μm³: DTI and tractography
Method	EPI 3D spin echo	EPI 3D spin echo	EPI 3D spin echo
Segments	1	1	1
TR/TE/SW	1000/26/357kHz	1000/28/357kHz	1000/26/357kHz
B-val	2800s/mm²	2800s/mm²	2800s/mm²
grad duration /separation	4.2/12ms	5.0/12ms	4.2/12ms
Amplitude (% of max)	64.26%	84.49%	64.26%
Diff orient	42 + 4	42 + 4	42 + 4
	Magnetization Transfer (MT) 250 μm³ : MTR
Method	FLASH 3D	FLASH 3D	FLASH 3D
TR/TE/flip/SW	47/2.6/13°/67kHz	47/2.6/15°/67kHz	47/2.6/13°/67kHz
MT Pulse shape/Power	Gauss/10μT	Gauss/10μT	Gauss/10μT
MT Offset	−1500 Hz/5 ppm	−1000Hz/5ppm	−1500 Hz/5 ppm
Number of pulses	3	3	3
Pulse length	12ms (228 Hz)	12ms (228 Hz)	12ms (228 Hz)