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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Sep 5;39(11):2353–2366. doi: 10.1161/ATVBAHA.119.312754

Figure 4. Wound Monocyte/Macrophages from Post-Septic Mice Demonstrate Decreased MLL1 and H3K4me3 at NFκB Binding Sites on Inflammatory Gene Promoters.

Figure 4.

A: Wound myeloid cells were isolated at day 3 postinjury from post-sepsis and sham control mice by MACS for CD11b+[CD3-CD19-Ly6G-] cells. ChIP analysis for H3K4me3 at Il1b, Il12, and Il23 promoter was performed (n=5/group). For all ChIP experiments, isotype control antibody to IgG was run in parallel. Dotted line represents isotype control. *p<0.05 by ANOVA test with Newman-Keuls Multiple Comparison test. B: Wound myeloid cells CD11b+[CD3-CD19-Ly6G-] were isolated from post-sepsis and control mice and Mll1 expression was quantified using qPCR (n=5/group). **p<0.01 by Student t test with Welch’s correction.