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. Author manuscript; available in PMC: 2020 Apr 23.
Published in final edited form as: Nature. 2019 Oct 23;574(7779):543–548. doi: 10.1038/s41586-019-1644-y

Extended Data Figure 9. Microglia in GF and ABX mice exhibit a developmentally immature phenotype.

Extended Data Figure 9.

a, Population frequencies and numbers of microglia in Ctrl and GF mice. b, Representative flow cytometry histogram and mean fluorescence intensity (MFI) of F4/80 staining on microglia from Ctrl and GF mice. c, Representative flow cytometry plots and population frequencies of CSF1R+ microglia in Ctrl and GF mice. d, Representative flow cytometry histogram and MFI of CSF1R expression gated on CSF1R+ microglia from Ctrl and GF mice. Data in a-d are representative of three independent experiments. n = 4/group. e, Population frequencies and numbers of microglia in Ctrl and ABX mice. f, Representative flow cytometry histogram and MFI of F4/80 staining on microglia from Ctrl and ABX mice. g, Representative flow cytometry plots and population frequencies of CSF1R+ microglia in Ctrl and ABX mice. h, Representative flow cytometry histogram and MFI of CSF1R expression gated on CSF1R+ microglia from Ctrl and ABX mice. Data in e are pooled from two independent experiments. n = 8/group. Data in f-h are representative of two independent experiments. n = 4/group. Data are mean ± SEM. Unpaired two-sided t tests were used. P values are indicated on the figure.