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. 2019 Oct 29;14(10):e0223753. doi: 10.1371/journal.pone.0223753

Fig 1. Outline of the experimental design.

Fig 1

In first experiment (A), zygotes were cultured in four culture conditions until the blastocysts stage: only in basic media (BM), BM supplemented with EGF, BM supplemented with HA and BM supplemented with EGF + HA. In the second experiment (B), preimplantation embryos were cultured in BM until the blastocyst stage (BM), or supplemented with EGF + HA after 4 cell stage (After_4C), after 16 cell stage (After_16C), until 4-cell (Until_4C), until 16-cell stage (Until_16C) or from zygote to the blastocyst stage (All_stages).