Fig 3. YitQ is an anti-toxin protein to the YIT toxin.
B. subtilis strains ΔsdpA-sdpR ΔyitR-yitM ΔsigW, Pspac-hy-yitQ ΔsdpA-sdpR ΔyitR-yitM ΔsigW, and Pspac-hy-sdpI ΔsdpA-sdpR ΔyitR-yitM ΔsigW were added to 2×SG agar containing 1 mM IPTG and poured into plates as lawns. Strains tested for antibiotic production (shown on the left of the figure as spots) were spotted on the lawns. Plates were incubated at 37°C. A growth inhibitory zone was observed if the lawn strain was sensitive to a compound produced by the strain spotted on it. Scale bar, 2 mm.