Video. This video demonstrates mechanical disaggregation of nanofat following the Tulip NanoTransfer protocol. For harvest, the abdominal region was infiltrated with tumescent fluid and adipose tissue was subsequently harvested. Aspirated adipose tissue (lipoaspirate) was washed with an equal volume of saline solution by hand rocking and removing the excess fluid. Dry lipoaspirate was mechanically disaggregated by a shearing process of transferring fat 30 times through 2.4-mm and 1.2-mm Luer-to-Luer devices, causing stroma fragmentation and a major adipocyte breakage. As a result, intracellular triglycerides released from broken adipocytes together with residual fluid present in lipoaspirate formed an emulsion containing stromal cells as aggregates. Mechanically disaggregated lipoaspirate was transferred through the NanoTransfer device to select for 0.6- to 0.4-mm size particles, generating nanofat as a final product.

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