Key resources table.
| Reagent type (species) or resources |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain (Mycobacterium tuberculosis) | H37Rv | ATCC | ||
| Strain (Mycobacterium tuberculosis) | Mtb-CS267 | Clinical Isolate | This study | |
| Cell Line (Homo sapiens) | Human Cord Blood (CB) purified CD34+ cells | STEMCELL Technologies | Catalog#70008.5 | Cell line maintained in StemSpan II Expansion Media - STEMCELL Technologies- Catalog#09605 |
| Cell (Homo sapiens) | Peripheral blood mononuclear cell (PBMC) | Cells maintened in RPMI 1640 complete, Sigma-Aldrich – Catalog#R8758 | ||
| Chemical Compound, drug | Middlebrook 7H10 agar | BD Biosciences | Catalog# 262710 | Supplemented with 10% Oleic Acid,Albumin, Dextrose, Catalase (Sigma-Aldrich – Catalog# M0678-1VL) |
| Chemical Compound, drug | L-Glutamine (200 mM) | Sigma-Aldrich | Catalog# 25030081 | |
| Chemical Compound, drug | Sodium Pyruvate (100 mM) | Life Technologies. | Catalog# 11360070 | |
| Biological Sample | Genomic DNA, Mycobacterium tuberculosis, Strain H37Rv | This study | Dosage: 10 μg/ml | |
| Chemical Compound, drug | Mycobacterium tuberculosis, Strain H37Rv, Purified Lipoarabinomannan (LAM) | BEI Resources | Catalog# NR-14848 | Dosage: 5 µg/mL |
| Peptide, Recombinant protein | Recombinant Human Interleukin 6 (rh IL-) | ImmunoTools | Catalog# 11340064 | |
| Antibody | FITC Anti-Lineage 1, human: CD3 clone SK7, CD16 clone 3G8, CD19 clone SJ25C1, CD20 clone L27, CD14 clone MφP9, CD56 clone NCAM. | BD Biosciences | Catalog# 340546 | (1:30) |
| Antibody | PE Anti-human CD34, clone 581. | BD Biosciences | Catalog# 555822 | (1:20) |
| Antibody | FITC Anti-human CD34, clone 8G12. | BD Biosciences | Catalog# 345801 |
(1:50) |
| Antibody | PerCP Anti-human CD34, clone 581. | Biolegend | Catalog# 343519 | (1:20) |
| Antibody | PECy7 Anti-human HLA-DR, clone L243 | Biolegend | Catalog# 307615 | (1:200) |
| Antibody | Bv510 Mouse Anti-Human HLA-DR, clone G46-6 | BD Horizon | Catalog# 563083 | (1:100) |
| Antibody | APC Anti-human CD38, clone HIT2 | Biolegend | Catalog# 303510 | (1:100) |
| Antibody | Bv421 Anti-Human CD64, clone 10.1. | BD Biosciences | Catalog# 562872 |
(1:100) |
| Antibody | FITC Anti-human CD10, clone HI10A | BD Biosciences | Catalog# 340925 |
(1:50) |
| Antibody | Alexa Fluor 488 Anti-human CD14, clone M5E2. | Biolegend | Catalog# 301817 | (1:50) |
| Antibody | APCCy7 Anti-mouse/human CD11b, clone M1/70 | Biolegend | Catalog# 101226 |
(1:100) |
| Antibody | V450 Anti-human CD14, clone MφP9 | BD Biosciences | Catalog# 560350 |
(1:100) |
| Antibody | PE Anti-human CD66b, clone G10F5. | Biolegend | Catalog#305106 | (1:100) |
| Antibody | APCCy7 Anti-human BDCA1, clone L161. | Biolegend | Catalog# 331520 |
(1:200) |
| Antibody | FITC Anti-human CD41a, clone 6C9. | ImmunoTools | Catalog# 21330413 |
(1:50) |
| Antibody | APC Anti-human BDCA2, clone 201A | Biolegend | Catalog# 354205 |
(1:50) |
| Antibody | Bv510 Anti-human BDCA3, clone 1A4. | BD Biosciences | Catalog# 563298 |
(1:100) |
| Antibody | PE Anti-human CD123, clone 7G3. | BD Biosciences | Catalog# 554529 |
(1:20) |
| Antibody | APC Anti-human CD16, clone 3G8. | BD BiosciencesCatalog# | 561248 | (1:50) |
| Antibody | V450 Anti-human CD64, clone 10.1. | BD Biosciences | Catalog# 561202 | (1:20) |
| Antibody | FITC Anti-human CD3, clone UCHT1. | Biolegend | Catalog# 300440 |
(1:100) |
| Antibody | FITC Anti-human CD19, clone 4G7. | BD Biosciences | Catalog# 347543 |
(1:50) |
| Antibody | Alexa Fluor 488 Anti-human CD14, clone M5E2. | BD Biosciences | Catalog# 561706 |
(1:50) |
| Antibody | PerCP-Cy5.5 Anti-human CD34, clone 8G12. | BD Biosciences | Catalog# 347203 |
(1:25) |
| Antibody | PE Anti-human IL-6, clone 8C9. | ImmunoTools | Catalog# 21670064 | (1:10) |
| Antibody | FITC Anti-human CD56, clone NCAM16.2. | BD Biosciences | Catalog# 345811 | (1:100) |
| Antibody | FITC Anti-human CD16, clone HI16a. | ImmunoTools | Catalog# 21810163 |
(1:100) |
| Antibody | Monoclonal Anti-STAT1 (phospo Y701), clone M135. | Abcam | Catalog# ab29045 | (1:1000) |
| Antibody | Monoclonal Anti-STAT1, clone SM1. | Abcam | Catalog# ab3987 | (1:1000) |
| Antibody | Polyclonal Anti-C/EBPβ | Santa Cruz Biotechnology | Catalog# sc-150 | (1:250) |
| Antibody | Neutralizing Anti-human IFNAR2, clone MMHAR-2 | PBL Assay Science | Catalog# 21370–1 | Dosage: 1 µg/mL |
| Antibody | Monoclonal, Anti-IFN-γ, clone B27. | ImmunoTools | Catalog# 21853531 | Dosage: 10 µg/mL |
| Antibody | Anti-IL6, Tocilizumab. | Roche | Dosage: 1 µg/mL | |
| Antibody | Monoclonal Anti-beta actin | Abcam | Catalog# mAbcam 8226 | (1:5000) |
| Chemical Compound, drug | Flexible Viability Stain 450 | BD Horizon | Catalog#562247 | (1:1000) |
| Chemical Compound, drug | Carbol Fuchsin | Sigma-Aldrich | Catalog# C4165 | |
| Chemical Compound, drug | Methylene Blue | Sigma-Aldrich | Catalog# 03978 | |
| Chemical Compound, drug | Hoechst 33342 | Immunochemistry technologies | Catalog# 639 | |
| Commercial assay, kit | M-PER Mammalian Protein Extraction Reagent | Thermo Fisher Scientific | Catalog# 78501 | |
| Commercial assay, kit | cOmplete ULTRA Tablets, Mini, EASYpack Protease Inhibitor Cocktail | Roche | Catalog# 05 892970001 | |
| Commercial assay, kit | High-Capacity cDNA Reverse Transcription Kit | Applied Biosystems | Catalog# 4368814 | |
| Chemical Compound, drug | TRIzol LS Reagent | Invitrogen | Catalog# 10296010 | |
| Commercial assay, kit | NuGEN - Trio low input RNA-seq | NuGEN | Catalog#0507–08 | |
| Software, algorithm | FlowJo software v. 10.1 | TreeStar | FlowJo, RRID:SCR_008520_ | https://www.flowjo.com/ |
| Software, algorithm | GraphPad Prism 6 Software | GraphPad | GraphPad Prism, RRID:SCR_002798 | https://www.graphpad.com/ |