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. 2019 Sep 23;129(11):4593–4608. doi: 10.1172/JCI120879

Figure 4. ANGPTL4 induces a rapid activation of RhoA and actomyosin contraction and a delayed activation of Rac1 and Cdc42.

Figure 4

(A–C) Activation of RhoA (A), Rac1 (B), and Cdc42 (C) over time in HUVECs exposed to 5 μg/mL rhANGPTL4. Positive controls: 0.5 U/mL thrombin (Rho assay); 1 μM sphingosine-1-phosphate (Rac1 and Cdc42 assays). Plus signs indicate positive controls. (D–G) Inhibition of 5 μg/mL rhANGPTL4-induced in vitro EC permeability by increasing doses of C. botulinum C3 toxin (0, 0.5, 2, 5 μg/mL) (D), HA1077 (0, 0.1, 1, 10 μM) (E), blebbistatin (0, 0.5, 5, 10 μM) (F), and ML7 (0, 10, 50, 100 μM) (G). One-way ANOVA (A–G). **P < 0.01; ***P < 0.001. Experiments were repeated at least 3 times.