Figure 2. Jmjd3 deficiency causes defects in T cell migration.

(A) Thymic CD4 SP cells from either WT or Jmjd3-cKO mice were purified by FACS, and equal numbers of cells were intravenously injected into sublethally irradiated Rag2^–/–γc^–/– mice (n = 5) (upper panel). Absolute numbers of CD4⁺ cells in the spleens of recipient Rag2^–/–γc^–/– mice were determined 24 hours after adoptive transfer (lower panel). (B) Thymic CD4 SP (TCRVα3.2⁺/Vβ11⁺) T cells from either 2D2:Jmjd3^fl/fl or 2D2:Jmjd3-cKO mice were purified by FACS, and equal numbers of cells were intravenously injected into sublethally irradiated C57BL/6 mice (n = 5). Absolute numbers of CD4⁺ TCRVα3.2⁺ TCRVβ11⁺ cells in the spleens and LNs of recipient C57BL/6 mice were determined 24 hours after adoptive transfer (left segment) and positive cell ratio (right segment) by FACS. Data are plotted as mean + SD from 3 independent experiments. *P < 0.05, **P < 0.01, Student’s t test.