Figure 6. Ca²⁺ dependency of the protective effects of recombinant annexin A6.

(A and B) Wild-type myofibers were isolated and loaded with a fluorescence marker of membrane damage, FM 1-43 (green). Myofibers were pretreated with recombinant annexin A6 (rANXA6) and then damaged in 1 mM Ca²⁺ solution or 0 mM Ca²⁺ plus EGTA, a calcium chelator. FM 1-43 fluorescence uptake over time was significantly reduced at 1 mM Ca²⁺ compared with when EGTA was present. Scale bars: 5 μm. Data are expressed as mean ± SEM. Differences were tested by 2-way ANOVA with Bonferroni’s multiple-comparisons test. *P < 0.05 (n = 10 myofibers per condition; n = 3 mice per condition).