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. 2019 Sep 23;129(11):4657–4670. doi: 10.1172/JCI128840

Figure 7. Local delivery using intramuscular injection of recombinant annexin A6 protected against muscle damage in vivo.

Figure 7

(A) Tibialis anterior muscles of wild-type mice were injected i.m. with recombinant human annexin A6 (rANXA6) or vehicle control and subsequently injured with cardiotoxin injection. (B) Gross imaging revealed decreased Evans blue dye (blue) uptake in rANXA6-pretreated muscle compared with the contralateral control muscle. (C) Immunofluorescence imaging revealed decreased dye uptake (red) in muscle pretreated with rANXA6. Surface plots of dye uptake depict reduced fluorescence in muscle pretreated with rANXA6. White dotted lines outline the muscle sections. (D) Tibialis anterior (TA) muscle pretreated with rANXA6 had a significant reduction, approximately 50%, of Evans blue dye fluorescence over muscle area compared with control muscle. Scale bars: 1 mm. Data are expressed as mean ± SEM. Differences were assessed by 2-tailed t test. *P < 0.05 (n = 3 mice per condition). EBD, Evans blue dye.