(A) IL-33 induced gene expression of the type 2 cytokines IL5 and IL13, and to a lesser extent IL4, in primary human CBMCs compared with unstimulated control (Ctrl), whereas IL-25, TSLP, HDM extract, or IL-13 did not (n = 4 per condition). *P < 0.0001 vs. Ctrl. (B) IL-33 induced production of IL-13 protein and to a lesser extent IL-5 protein in LUVA mast cells (n = 2 per condition at 3 time points). Differences between multiple conditions were assessed by 1-way ANOVA with correction for multiple comparisons. Shown are mean values and SEM bars. *P < 0.0001.