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. 2019 Oct 14;129(11):4979–4991. doi: 10.1172/JCI126402

Figure 7. Mast cells regulate the epithelial expression of IL33 in a feed-forward loop.

Figure 7

In a coculture system with differentiated epithelial cells cocultured with or without mast cells for 48 hours, priming of the mast cells with IL-33 significantly amplified the epithelial expression of IL33. Stimulation of the epithelial surface with HDM further amplified IL33 expression. Similar results were identified for LAD2 mast cells (n = 3 per condition) (A), LUVA mast cells (n = 3 per condition) (B), and primary CBMCs (n = 2 per condition) (C). (D) IL-33 protein was detected in the basolateral media only when IL-33–primed mast cells were added to the coculture system in which HDM was added to the apical surface (n = 4 per condition). IL-33 protein was not detectable when IL-33–primed mast cells that were similarly exposed to HDM were cultured in the absence of the epithelium. Differences between multiple conditions were assessed by 1-way ANOVA with correction for multiple comparisons. Shown are mean values and SEM bars.