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. 2019 Oct 14;129(11):4962–4978. doi: 10.1172/JCI127959

Figure 1. Mitochondrial Rac1 activity is increased in IPF BAL cells.

Figure 1

Immunoblot analysis of Rac1 expression in isolated (A) mitochondria or (B) cytosol fractions from BAL cells obtained from healthy subjects (n = 4) or patients with IPF (n = 4). (C) Mitochondrial Rac1 activity in BAL cells from healthy subjects (n = 6) or patients with IPF (n = 5). BAL cells from healthy subjects (n = 6–7) or patients with IPF (n = 6–8) were analyzed for mRNA expression of (D) ARG1 and (E) PDGFB. (F) Arginase 1 activity in BAL cells from healthy subjects (n = 5) or patients with IPF (n = 8). (G) Rac1 activity in BAL cells isolated at the indicated time points from mice exposed to saline or bleomycin (n = 3–4 mice/time point). Immunoblot analysis of Rac1 expression in isolated (H) mitochondria or (I) cytosolic fractions from BAL cells obtained from mice treated with saline (n = 4) or bleomycin (n = 5). (J) Mitochondrial Rac1 activity in BAL cells from saline- (n = 5) or bleomycin-exposed (n = 5) mice. (K) PDGF-BB and (L) IL-10 were measured in BALF from saline- or bleomycin-exposed mice at the indicated time points (n = 3–4 mice/time point). Values indicate the mean ± SEM. *P < 0.05, **P < 0.001, and ***P < 0.0001, by 2-tailed Student’s t test (C–F and J) and 2-way ANOVA with Bonferroni’s post test (G, K, and L).