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. 2019 Oct 14;129(11):4962–4978. doi: 10.1172/JCI127959

Figure 6. Rac1 is required for GGOH- and bleomycin-induced lung fibrosis.

Figure 6

Ten days after exposure of WT and Rac1–/– Lyz2-Cre mice to saline or bleomycin, pumps containing vehicle or GGOH were s.c. implanted, and the mice were sacrificed 11 days later. (A) Total number of BAL cells and (B) cell differential (n = 5/group). Mac, macrophage; PMN, polymorphonuclear. (C) Mitochondrial Rac1 activity (n = 5/group). Inset in C shows Rac1 immunoblot analysis of BAL cells. (D) mtROS generation in isolated BAL cells (n = 5–6/group). (E) Active TGF-β1, (F) PDGF-BB, and (G) Ym-1 expression in BALF (n = 4–5/group). (H and I) Ten days after exposure of WT mice to saline or bleomycin, daily i.p. injections of simvastatin (SIMV) (20 mg/kg/day) were administered, and mice were sacrificed 11 days later. (H) Active TGF-β1 and (I) PDGF-BB expression in BALF (n = 4/group). (J) Representative images of lung histology with Masson’s trichrome staining (n = 5/group). Original magnification, ×2.5. (K) Hydroxyproline content (n = 5/group). Values indicate the mean ± SEM. **P < 0.001 and ***P < 0.0001, by 1-way ANOVA followed by Tukey’s multiple comparisons test.