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Time courses of dialysate acetylcholine (Ach) concentrations elicited by intracerebroventricular injection of ghrelin (●) or vehicle (○) (n = 5 in each group). Data are concentrations in dialysate samples collected over 20-minute durations. Values are mean ± SE. ‡P < 0.01 vs. baseline before injection. *P < 0.05, ghrelin vs. vehicle. Adapted from Shimizu et al.22)
