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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Hepatology. 2019 Jun 21;70(5):1506–1520. doi: 10.1002/hep.30699

Figure 3. IFITMs differentially restrict HCV variants isolated from patients undergoing liver transplantation.

Figure 3

Huh7.5.1 cells were transduced to express the indicated IFITM proteins and then infected with HCVpp pseudotyped with HCV E1E2 patient variants isolated from patients undergoing liver transplantation. Infection was assessed after 72 h by measuring luciferase activity. (A) Results for variants from two patients are expressed as means ± SEM percentage HCVpp infection compared to control cells (set at 100%) from three independent experiments performed in triplicate. (B) TCID50 analysis of HCVcc infection of IFITM-expressing Huh7.5.1 cells. Shown are means of three experiments performed in sextuplicates. Error bars represent SEM. Control was set to 100 %. (C and D) Each dot represents the result for a single variant. Results for % Infection of IFITM2 (C) and IFITM3 (D) positive cells compared to the control of HCV variants from five different patients. *P < 0.05; ** P < 0.01 one-sided Students T-test. Shown are the means of three experiments performed in triplicates.