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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Hepatology. 2019 May 23;70(5):1582–1599. doi: 10.1002/hep.30693

FIGURE 7. IL-1 receptor signaling contributes to metastatic liver tumor growth in the NAFLD condition.

FIGURE 7.

WT mice were injected via the spleen with MC38 colorectal cancer (CRC) cells after 6 weeks of low-fat diet (LFD) or high-fat diet (HFD) which was continued for an additional 2 weeks. A subset of mice were treated with recombinant IL-1 receptor antagonist (IL-1Ra, 10 mg/kg body weight) s.c. daily. (A, left) Macroscopic appearance of the liver. Arrow, tumors. (A, right) Liver weight. (B, left) Number of visible liver tumors. (B, right) Maximal size of tumors. (C) Oil red O staining and its quantification. n=7–10, each group. Representative pictures are shown. Data are shown as mean ± S.D. per group. (D,E) TAMs (D) and M2 macrophages (E) were examined by immunohistochemistry (IHC) for F4/80 and CD206, respectively. The F4/80- or CD206-positive area in tumors was quantified by ImageJ. n=7–10, each group. T, tumor. Representative pictures are shown. Data are shown as mean ± S.D. per group.