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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Hepatology. 2019 Jun 22;70(5):1674–1689. doi: 10.1002/hep.30706

Figure 1.

Figure 1

TGFβ Drives Expression of an HSC-Activating Gene Program in Cholangiocytes.

A. Heat Map representing the gene changes in a cholangiocytes cell line (HIBEC) treated with 10ng/ml TGFβ for 48 hours (green: upregulated gene set; red: downregulated gene set). n=3

B. Ingenuity Pathway Analysis (IPA) shows Integrin signaling and Hepatic Fibrosis/Hepatic Stellate Cell Activation as the most significantly upregulated pathways (inset shows 10 representative genes from the Heaptic Stellate Cell Activation Pathway).

C. RT-PCR in cholangiocytes treated with 10ng/ml TGFβ for 24 hours confirms upregulation of HSC activation genes. *p<0.01 and **p<0.001. All error bars are SEM, n=3

D. EnrichR analysis identified EZH2 as an epigenetic regulator of the cholangiocyte TGFβ response (p-vaue represents Fisher exact test).