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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: J Neurochem. 2019 Oct 13;151(3):279–288. doi: 10.1111/jnc.14855

Fig. 4.

Fig. 4.

GRABDA can detect both the release and modulation of dopamine evoked by optogenetic stimulation. The red-activated excitatory opsin C1V1 was expressed in the substantia nigra pars compacta (SNc) and used to optically activate dopaminergic neurons, while GRABDA1m was expressed in the striatum (Str) and used to measure dopamine. The GRABDA1m fluorescence signal was prolonged by application of the dopamine transporter blocker methylphenidate and blocked by application of the D2R-specific antagonist Etic. tdTomato was co-expressed with GRABDA1m and imaged simultaneously in order to rule out any possible artifacts. Figures are modified from original research paper(Sun et al. 2018) with permission from the publisher.