Skip to main content
. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Ann Biomed Eng. 2019 Apr 29;48(3):992–1005. doi: 10.1007/s10439-019-02279-0

Figure 7.

Figure 7.

New bone formation at four weeks post-implantation. Toluidine blue (TB) staining is present in all panels for orientation purposes. (A) Overall representation of entire calavaria for both PLGA and hybrid matrices for detection of mineralization (white) and cellular GFP (green) signal. Box 1 is enlarged and shown in panels B-E and Box 2 in panel F. (B) Enlarged view of boxed portion of A for hybrid matrix. (C) Overlay of AC (alizarin complexone) (red) and GFP signal. (D) Overlay of AP (alkaline phosphatase) (also red) and GFP. (E) Overlay of TRAP staining (tartrate resistant acid phosphatase) (yellow) and GFP. (F) High magnification image of overlay of GFP and HuNuc Ag (human nuclear antigen).