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. 2019 Oct 28;16:121. doi: 10.1186/s12985-019-1232-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Histopathological and immunohistochemistry analyses. a–d Comparison of small intestinal lesions in different groups (Mock, P120, P64, and P10) at necropsy at 3 days post infection (dpi). e–h H&E-stained jejunum tissue sections of the different groups at 3 dpi. i–l h&e-stained ileum tissue sections of the different groups at 3 dpi. m–p Immunohistochemically stained jejunum tissue sections of the different groups at 3 dpi PEDV antigen is indicated by arrows). q–t Immunohistochemically stained ileum tissue sections of the different groups at 3 dpi (PEDV antigen is indicated by arrows). P10, PEDV after 10 passages; P64, PEDV after 64 passages; and P120, PEDV after 120 passages; h&e, hematoxylin and eosin; PEDV, porcine epidemic diarrhea virus