Table 3.
Differentially abundant proteins identified with high confidence (defined in Section The Apoplastic Proteome During the Biotrophic Phase) from both comparisons. All were queried against the nonredundant database of NCBI using the pBLAST algorithm to help identify unknown proteins as far as possible through homology.
| Time | Protein IDs | Putative identity | Peptidesa | Scoreb | pBLAST return |
|---|---|---|---|---|---|
| 48 h | A0A1D6D5D4 | WHEAT plastocyanin | 4 | 317.12 | Plastocyanin, chloroplastic |
| A0A1D6RL92 | WHEAT uncharacterized protein | 14 | 323.31 | Loricrin-like | |
| A0A1D5V896 | WHEAT uncharacterized protein | 9 | 127.54 | Glucan endo-1,3-beta-glucosidase, acidic isoform-like | |
| A0A0C4BK99 | WHEAT uncharacterized protein | 6 | 43.582 | 60S ribosomal protein l35a-1-like | |
| W5C8U5 | WHEAT peroxidase | 9 | 263.07 | Peroxidase 1-like | |
| C6ETB3 | WHEAT peroxidase | 10 | 313.09 | Class III peroxidase | |
| Q9XEN5 | WHEAT beta-1,3-glucanase | 12 | 323.31 | Beta-1,3-glucanase precursor | |
| A0A1D5TI66 | WHEAT uncharacterized protein | 6 | 172.8 | Ervatamin-C-like | |
| Q1ERG1 | WHEAT endo-beta-1,3-glucanase | 11 | 323.31 | Endo-beta-1,3-glucanase | |
| O82716 | WHEAT glucan endo-1,3-beta-D-glucosidase | 17 | 281.7 | Glucan endo-1,3-beta-D-glucosidase | |
| Q94F73 | WHEAT pathogenesis-related protein 1 | 9 | 323.31 | Pathogenesis-related protein PRB1-3 | |
| A0A1D6BJ70 | WHEAT uncharacterized protein | 12 | 263.67 | Aspartyl protease family protein At5g10770-like | |
| A0A1D5YUG0 | WHEAT uncharacterized protein | 4 | 209.67 | Thaumatin-like pathogenesis-related protein 3 | |
| C3UZE5 | WHEAT pathogenesis-related protein 1-1 | 7 | 293.7 | Pathogenisis-related protein 1.1 | |
| A0A1D5TQY9 | WHEAT peroxidase | 11 | 323.31 | Peroxidase 1 | |
| O82714 | WHEAT pathogenesis-related protein 1-3 | 8 | 323.31 | Pathogenisis-related protein 1.1 | |
| A0A0H4TM98 | WHEAT chitinase | 8 | 107.19 | Chitinase | |
| A0A1D5SA13 | WHEAT uncharacterized protein | 3 | 323.31 | Chitinase 8-like | |
| Q41584 | WHEAT thaumatin-like protein | 3 | 323.31 | Thaumatin-like protein | |
| 72 h | A0A1D5SU87 | WHEAT uncharacterized protein | 22 | 323.31 | Chitinase 8-like |
| Q41584 | WHEAT thaumatin-like protein | 3 | 323.31 | Thaumatin-like protein | |
| F8S6U9 | WHEAT pathogenesis-related protein 1-19 | 5 | 105.85 | Pathogenesis-related protein 1-19 | |
| W5C8U5 | WHEAT peroxidase | 9 | 323.31 | Peroxidase 1-like | |
| A0A1D5V896 | WHEAT uncharacterized protein | 10 | 323.31 | Glucan endo-1,3-beta-glucosidase, acidic isoform-like | |
| D8L9Q2 | WHEAT glucan endo-1,3-beta-glucosidase GII | 14 | 323.31 | Glucan endo-1,3-beta-glucosidase GII precursor | |
| Q9XEN5 | WHEAT beta-1,3-glucanase | 12 | 323.31 | Beta-1,3-glucanase precursor | |
| Q4JK90 | WHEAT beta-1,3-glucanase | 18 | 323.31 | Beta-1,3-glucanase | |
| O82716 | WHEAT glucan endo-1,3-beta-D-glucosidase | 18 | 323.31 | Glucan endo-1,3-beta-D-glucosidase | |
| C6ETB3 | WHEAT peroxidase | 11 | 323.31 | Class III peroxidase | |
| A0A1D5UH99 | WHEAT uncharacterized protein | 10 | 152.63 | Chitinase 5-like | |
| O82714 | WHEAT pathogenesis-related protein 1-3 | 11 | 97.119 | Pathogenisis-related protein 1.1 | |
| A0A1D5W1T2 | WHEAT uncharacterized protein | 13 | 323.31 | Glucan endo-1,3-beta-glucosidase GIII-like | |
| Q9SQG3 | WHEAT PR-4 (fragment) | 6 | 211 | PR-4, partial | |
| Q1ERG2 | WHEAT endo-beta-1,3-glucanase | 9 | 200.56 | Endo-beta-1,3-glucanase | |
| A0A1D5TQY9 | WHEAT peroxidase | 11 | 323.31 | Peroxidase 1 | |
| A0A1D6BV27 | WHEAT uncharacterized protein | 13 | 323.31 | PR17d precursor | |
| A0A1D5SA13 | WHEAT uncharacterized protein | 3 | 322.23 | Chitinase 8-like | |
| Q43212 | WHEAT peroxidase | 10 | 323.31 | Peroxidase | |
| W5DDM8 | WHEAT uncharacterized protein | 8 | 298.38 | Somatic embryogenesis receptor kinase 2-like | |
| C3UZE5 | WHEAT pathogenesis-related protein 1-1 | 12 | 323.31 | Pathogenisis-related protein 1.1 | |
| A0A1D5TI66 | WHEAT uncharacterized protein | 7 | 294.77 | Ervatamin-C-like | |
| A0A1D5V895 | WHEAT uncharacterized protein | 11 | 225.1 | Glucan endo-1,3-beta-glucosidase GII precursor, putative, expressed | |
| A0A1D5YUG0 | WHEAT uncharacterized protein | 4 | 238.3 | Thaumatin-like pathogenesis-related protein 3 | |
| A0A1D6BC87 | WHEAT uncharacterized protein | 10 | 117.77 | ||
| A0A077S0N0 | WHEAT uncharacterized protein | 12 | 323.31 | Glucan endo-1,3-beta-glucosidase, acidic isoform-like | |
| Q1ERG1 | WHEAT endo-beta-1,3-glucanase | 11 | 323.31 | Endo-beta-1,3-glucanase | |
| A0A1D6BJ70 | WHEAT uncharacterized protein | 12 | 323.31 | Aspartyl protease family protein At5g10770-like | |
| A0A1D5X5E7 | WHEAT uncharacterized protein | 9 | 323.31 | Thaumatin-like protein TLP8 | |
| A0A1D5WHM8 | WHEAT uncharacterized protein | 12 | 148.36 | Glucan endo-1,3-beta-glucosidase GIII-like | |
| A0A1D5V4E2 | WHEAT uncharacterized protein | 16 | 43.778 | Glucan endo-1,3-beta-glucosidase GII-like |
a
The number of peptides observed.
b
Andromeda score, defined by Tyanova et al. (2016a). All of the proteins reported here scored above the threshold.