Table 2.
Antibodies for Western blot (WB), immunohistochemistry (IHC) and immunofluorescence (IF) analyses
| Antibody | Company | Catalog # | Dilution (WB/ IHC/ IF) |
|---|---|---|---|
| α-tubulin | Sigma-Aldrich | T6199 | 1:1000/ X/ X |
| β-tubulin | Abcam | ab6046 | X/ X/ 1:200 |
| β-actin | Abcam | ab8227 | 1:1000/ X/ X |
| Claudin-5 (rb) | Thermo Fisher Scientific | 34–1600 | 1:500/ 1:200/ 1:200 |
| Claudin-5 (ms) | Invitrogen | 35–2500 | X/ X/ 1:200 |
| Zonula-Occludens-1 | Thermo Fisher Scientific | 61–7300 | 1:500/ 1:500/ 1:200 |
| Occludin | Thermo Fisher Scientific | 71–1500 | 2 μg/mL/ 1:50/ X |
| Occludin | Invitrogen | OC-3F10 | X/ X/ 1:200 |
| VE-Cadherin | Santa Cruz | Sc-6458 | 1:500/ 1:200/ 1:200 |
| Tie-2 | R&D | AF762 | 0.1 μg/mL/ 1:200/ X |
| pTie-2 | R&D | AF2720 | 0.5 μg/mL/ 2.5 μg/mL |
| ABCC4 | Cell signaling | 12857S | 1:1000/ X/ X |
| wide-spectrum CK | Abcam | ab9377 | X/ 1:20/ X |
| anti-mouse IgG | Dianova | 115–035-146 | X/ 1:500/ X |
| GFAP | Dako | ZO334 | X/ X/ 1:5000 |
| Iba1 | Wako | 019–19,741 | X/ X/ 1:500 |
| Ang2 | Thermo Scientific | PA5–27297 | 1:5000/ 1:800/ X |
| CD31 (rt) | Dianova | DIA-310 | X/ X/ 1:200 |
| DAPI | invitrogen | D1306 | X/ X/ 1:1000 |
ms mouse, rb rabbit, rt rat