Fig. 6.

Mast cell supernatant activates the JAK-STAT signaling pathway in HUVECs. a HUVECs treated with supernatants from mast cells stimulated with SCF or A549 cell-derived exosomes were analyzed via Western blotting and were compared with HUVECs treated with medium and tryptase. Phosphorylated (p)-JAK and p-STAT and total JAK and STAT were measured by Western blotting and protein loading was normalized, with the samples also probed for GAPDH. b and c Relative intensity was calculated for p-JAK and p-STAT. All of the above data are representative of three independent experiments (n = 3). *P < 0.05 and **P < 0.01