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. 2019 Oct 28;11:149. doi: 10.1186/s13148-019-0732-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Analysis of 5-mCpG and 5-hmCpG content at single-CpG resolution of the Dao regulatory region in the cerebellum. Bar plots represent methylation (black) and hydroxymethylation (line pattern) levels at individual CpG site in Dao regulatory region at the P1 to P15 stages. Average 5-hmC levels was calculated by subtracting values obtained from sequence analysis of DNAs treated with an oxidant reagent plus sodium bisulfite to the average methylation values obtained from DNA treated with bisulfite only. For each time point, results are indicated as the mean of n = 3 mice