Fig. 6.

DNA methylation at the Srr locus and Srr gene expression. a The analyzed CpG island in the Srr promoter region is shown. White box represents the putative upstream regulatory region; black box indicates the first exon; and gray box indicates the first intron. The analyzed Srr amplicon is located between the black arrows on the top, indicating the forward and reverse primers used for bisulfite amplification. The positions of the analyzed CpGs refer to the TSS (+ 1). The Srr sequence was retrieved by Ensembl with the accession number: ENSMUSG00000001323. b Average methylation (%) at single-CpG level is indicated for each brain region during ontogenesis. c The average methylation (%) of the Srr gene in all brain regions at all analyzed developmental stages is reported. Statistical analyses were performed using one-way ANOVA followed by Tukey’s multiple comparison post-hoc test. Alpha was considered significant at ≤ 0.001. d The spatiotemporal distribution of Srr mRNA levels is reported in histograms. Srr mRNA expression is normalized to the mean values for two housekeeping genes and expressed as 2^–∆Ct values. Statistical analyses were performed using one-way ANOVA followed by Tukey’s multiple comparison post-hoc test. * p ≤ 0.001