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. 2019 Oct 29;18:150. doi: 10.1186/s12943-019-1076-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Knockdown of circMYO10 inhibits osteosarcoma cell proliferation and migration, and induces cell cycle arrest. a Transwell migration and invasion assays demonstrated that downregulation of circMYO10 compromises the cell migration and invasion abilities in MG63 and U2OS cells. Data represents the mean ± SD (n = 3). Scale bars = 50 μm. b CircMYO10 knockdown suppresses cell migration in MG63 and U2OS cells as evaluated by a wound healing assay. Data represents the mean ± SD (n = 3). Scale bars = 200 μm. c CircMYO10 knockdown inhibits colony formation in both MG63 and U2OS cells. Data represents the mean ± SD (n = 3). d Knockdown of circMYO10 inhibited cell proliferation as indicated by CCK-8 assays in MG63 and U2OS cells. Data represents the mean ± SD (n = 18). e Downregulation of circMYO10 arrested MG63 and U2OS cells at the G0/G1 phase. Data represents the mean ± SD (n = 3). f CircMYO10 knockdown inhibited anchorage-independent colony formation of both MG63 and U2OS cells. Scale bars = 50 μm. g The protein expression of N-cadherin, E-cadherin, Vimentin, and cyclinD1 was detected by western blot analysis in both MG63 and U2OS cells transfected with SicircMYO10. Three independent assays were performed in the above assays. a-e * P < 0.05, ** P < 0.01, *** P < 0.001 (Student’s t-test)