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. 2019 Oct 28;38:429. doi: 10.1186/s13046-019-1400-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — miR-133a-3p promotes breast cancer cells proliferation and stemness in vitro. a-c Proliferation of MCF-7 cells (a), MDA-MB-468 cells (b) and MDA-MB-231 cells (c) transfected with mimics NC, miR-133a-3p mimics, inhibitor NC, or miR-133a-3p inhibitor detected by CCK8 assay. d-f The percentage of CD44+/CD24- population in MCF-7 cells (d), MDA-MB-468 cells (e), and MDA-MB-231 cells (f) transfected with mimics NC, miR-133a-3p mimics, inhibitor NC or miR-133a-3p inhibitor. g-i The protein levels of pluripotent transcription factors in MCF-7 cells (g), MDA-MB-468 cells (h), and MDA-MB-231 cells (i) transfected with mimics NC, miR-133a-3p mimics, inhibitor NC, or miR-133a-3p inhibitor by western blotting. *P < 0.05; **P < 0.01; ***P < 0.001