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PecA does not affect LipY surface localization and lipase activity. (A) Surface localization of LipY was measured on whole cells by flow cytometry for M. marinum M wild-type and ΔpecA strains expressing LipYtub. LipYtub was detected via its C-terminal HA tag by anti-HA antibody (10,000 bacteria were counted per condition). (B) Lipase activity was measured by the fluorescently based DGGR assay. Standard deviation values are shown (n = 3). a.u., arbitrary units; n.s., not significant.
