TABLE 3.
Strain or plasmid |
Description | Source or reference |
---|---|---|
Strains | ||
UA159 | S. mutans type strain | ATCC 700610 |
UA159ΔSMU_308 | Scarless knockout of sorbitol-6-phosphate 2-dehydrogenase (SMU_308); deleted bases 294120–297024 |
This study |
UA159ΔgalKT | Scarless knockout of galactokinase (galK, SMU_886) and galactose-1-phosphate uridylyltransferase (galT, SMU_887); deleted bases 838314–842938 |
This study |
Plasmids | ||
pDL278 |
E. coli/streptococcal shuttle vector; Specr |
67 |
pSMU308 | pDL278::SMU_308; SMU_308 includes the native promoter |
This study |
pGalKT | pDL278::galKT; galKT includes the native promoter |
This study |